Draft genome sequence data of Bifidobacterium longum strain VKPM Ac-1636, a prospective probiotic isolated from human gut.

Data on the draft genome sequence of Bifidobacterium longumsubsp. longum strain Ac-1636 is presented in this report. This strain, isolated from the digestive tract of one-year old healthy infant, was deposited in the Russian National Collection of Industrial Microorganisms as a prospective candidate for development of probiotics and probiotic foods. The 2,321,741 bp draft genome consists of 73 scaffolds with N50 of 162,253 bp. Genome annotation revealed the presence of multiple determinants of probiotic properties of this strain. The draft genome sequence data of strainAc-1636 is available in DBJ/EMBL/GenBank under the accession nos. RZHL00000000, PRJNA511803 and SAMN10644101 for Genome, Bioproject and Biosample, respectively.

Data on draft genome sequence of Bacillus sp. strain VKPM B-3276 isolated from Culex pipiens larvae.

The draft genome sequence of Bacillus sp. VKPM B-3276, a mesophilic, gram-positive bacterium, isolated from dead Culex pipiens larvae is presented. This strain was deposited in the Russian National Collection of Industrial Microorganisms as a prospective candidate for development of new entomopathogenic agents. The genome of Bacillus sp. VKPM B-3276 was 6,126,346 bp in length with predicted completeness of 99.43%. Genome analysis identified 6518 protein-coding sequences and 111 RNAs genes. 13% (271) of the protein-coding genes were assigned to "Carbohydrates" subsystem category, according to RAST/SEED. Among them about 50 enzymes, responsible for chitin, peptidoglycan and related molecules decomposition, were found. The draft genome of strain VKPM B-3276 was deposited at DBJ/EMBL/GenBank under the accession nos. RZHM00000000, PRJNA511803 and SAMN10644103 for Genome, Bioproject and Biosample, respectively.

Aim23p Interacts with the Yeast Mitochondrial Ribosomal Small Subunit.

Protein synthesis in mitochondria is generally organized in a bacterial-like manner but, at the same time, possesses several unique traits. Translation initiation in mitochondria is regulated by two protein factors, mtIF2 and mtIF3. Previously we demonstrated that Saccharomyces cerevisiae Aim23 protein is an ortholog of IF3 in budding yeast. However, the data on the interactions between Aim23p and other proteins are limited. Here, we demonstrated that Aim23p interacts with the yeast mitochondrial ribosomal small subunit both in vivo and in vitro using co-immunoprecipitation and density gradient sedimentation.